Monitoring of Protein Expression in Gene & Cell therapy

Gene therapy is based on the administration of genetic material into living cells to modify and manipulate the expression of a gene. This modification can lead to different mechanism such as gene inactivation or expression of new specific proteins. Different vectors can be used, the most common ones are plasmid DNA and viral vectors (Adeno-Associated-Virus, AAV), bacterial vector, patient-derived cellular gene therapy product (combine gene and cell therapy). To optimize and monitor the therapy efficacy, but also the safety one, it is mandatory to perform biological analysis. While the characterization of DNA and RNA can be performed by qPCR, specific expressed proteins analysis is also mandatory.

To support you through your R&D and quality control analysis, ANAQUANT developed different analytical approaches to identify and quantify your proteins of interest.
Identification and quantification of proteins of interest will help you for:
Expressed proteins sequence validation.
Relative and absolute quantification for samples comparison.
Identify and quantify protein impurities for Batch to Batch comparison in CMC.