Anaquant HCP analysis I Protein characterisation I Protein analysis
Post-translational modifications (PTM) are known to impact protein activities and biochemical pathways. Phosphorylation is one of the most prevalent protein modifications involved in numerous cellular processes and concerned several diseases.
In this case study, because one of the symptoms of the Alzheimer disease is the hyperphosphorylation of TAU protein, we did a multiplex analysis of the phosphorylation sites locations of this protein to validate the efficacy of a specific treatment and understand the biological mechanism of a drug candidate on phosphorylation sites.
Due to the fact that 20% of TAU protein sequence is composed by potential phosphorylation sites, LC-MS has been chosen to perform the study. After enzymatic digestion on samples containing different version of the protein, we were able to specifically detect all different phosphorylated sites on a specific peptide. The method development has been implemented on mouse samples to detect the phosphorylation evolution on Wild Type and Knock out mouse.
Thanks to this method development, scientists could be able to:
- Quickly determine the efficacy of the drug
- Select the best animal models
- Understand the biological mechanism associated to the disease and the drug effects.
- Support the Critical Quality Attributes (CQA)